Effect of Aframomum melegueta Seed Extract on Castor Oil-Induced Diarrhea

Tagged:  

GRAINS of paradise
AFRAMOMUM
PLANTS, Edible
ZINGIBERACEAE
ZINGIBERALES
CASTOR oil
LAXATIVES
VEGETABLE oils

Abstract:The effect of the aqueous (aq) seed extract of Aframomum melegueta K. Schum. (Zingiberaceae) on castor oil-induced diarrhea, intestinal fluid secretion, and gastrointestinal transit was investigated in the study. Castor oil (10 ml/kg, p.o.) induced copious diarrhea in all rats 3h after treatment. Furthermore, it produced a significant increase in the volume of intestinal fluid secretion in rats and also enhanced intestinal transit in mice. The aq seed extract of A. melegueta (100–500 mg/kg, p.o.) offered significant protection against diarrhea induced by the oil. At a dose range of 250–500 mg/kg, the extract reduced significantly the volume of fluid secretion in castor oil-treated rats. At these doses, it also demonstrated a significant antitransit activity in a dose-related manner. Acetylsalicylic acid (100 mg/kg, p.o.) delayed diarrhea and reduced the number of animals with diarrheal droppings to 20%. At the same dose level, acetylsalicylic acid reduced significantly the volume of intestinal fluid secretion but lacked antitransit property in castor oil-treated animals. N-Nitro-L-arginine methyl ester (L-NAME) (2.5–10 mg/kg, i.p.) dose-dependently reduced the number of animals with diarrhea. At 50 mg/kg i.p., it offered 100% protection against diarrhea induced by the oil. Furthermore, L-NAME (10 mg/kg, i.p.) significantly inhibited both the intestinal fluid secretion and gastrointestinal transit induced by castor oil. However, L-NAME (10 mg/kg, i.p.) did not significantly modify the antidiarrheal effect of A. melegueta. L-Arginine, a substrate of nitric oxide synthase or isosorbide dinitrate, a nitric oxide donor, did not alter the effect of A. melegueta on diarrhea. Ascorbic acid (100 mg/kg, p.o.) and ?-tocopherol (20 mg/kg, p.o.) reduced the number of animals with diarrhea to 80% and 70%, respectively. However, they both lacked significant activities on intestinal fluid secretion and gastrointestinal transit induced by castor oil....

Share this with your friends
AttachmentSize
Effect of Aframomum melegueta Seed Extract on Castor Oil-Induced Diarrhea.pdf411.64 KB